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Biochemistry

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JoVE Journal Biochemistry
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
 

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Article DOI: 10.3791/55448-v • 13:28 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1, 1, 2, 2, 2, 1, 1
1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, 2Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston

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This paper presents a protocol for processing cryo-EM images using the software suite SPHIRE. The present protocol can be applied for nearly all single particle EM projects that target near-atomic resolution.

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High-resolution Single Particle Analysis Electron Cryo-microscopy SPHIRE Macromolecular Complexes Image Processing Software Automatic Validation Mechanism Cryo-EM Data User-friendly Near-atomic Resolution Complex Micromolecular Machines Sample Quality Input Data SPHIRE Application GUI UTILITIES Cryo-microscopy Image E2display Measurement Tool Angstrom Per Pixel Project Settings CTF Window Size Particle Box Size Particle Diameter
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