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Developmental Biology

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JoVE Journal Developmental Biology
Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
 

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

Article DOI: 10.3791/55587-v • 11:13 min • May 12th, 2017
May 12th, 2017 •
1, 1, 1, 1,2, 1
1Department of Anatomy and Embryology, Leiden University Medical Center, 2Department of Applied Stem Cell Technologies, University of Twente

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Electrophysiological characterization of cardiomyocytes derived from human Pluripotent Stem Cells (hPSC-CMs) is crucial for cardiac disease modeling and for determining drug responses. This protocol provides the necessary information to dissociate and plate hPSC-CMs on multi-electrode arrays, measure their field potential, and a method for analyzing QT and RR intervals.

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Electrophysiological Analysis Human Pluripotent Stem Cell-derived Cardiomyocytes HPSC-CMs Multi-electrode Arrays MEAs Electrical Signal Field Potential Dissociation Plate Measure Quantify Duration Frequency Mutations Controls Advantages Drug Screening Patient Cells Visual Demonstration Clean Chips Sterile Petri Dish Distilled Water Humidified Chamber Culture Medium PolyTetraFluoroEthylene Rings
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