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Biochemistry

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JoVE Journal Biochemistry
Oligopeptidkonkurrenceanalyse for phosphoryleringsstedbestemmelse
 
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Oligopeptidkonkurrenceanalyse for phosphoryleringsstedbestemmelse

Article DOI: 10.3791/55708-v • 09:16 min • May 18th, 2017
May 18th, 2017 •
1, 1, 2, 2, 1
1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University

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Peptidkonkurrenceanalyser anvendes i vid udstrækning i en række molekylære og immunologiske eksperimenter. Dette papir beskriver en detaljeret fremgangsmåde til et in vitro oligopeptidkompeterende kinaseassay og de tilhørende valideringsprocedurer, som kan være nyttige til at finde specifikke phosphoryleringssteder.

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Biochemistry udgave 123, peptidinhibitor phosphorylering AMPK Nrf2 konsensusmotiv site-directed mutagenese
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