Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Expansion and Adipogenesis Induction of Adipocyte …
JoVE Journal
Developmental Biology

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Developmental Biology
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
 

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Article DOI: 10.3791/55818-v • 08:28 min • June 30th, 2017
June 30th, 2017 •
1, 2, 2, 1
1Department of Large Animal Clinical Sciences, Michigan State University, 2Department of Pharmacology and Toxicology, Michigan State University

Chapitres

résumé
Automatic Translation

Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).

Tags

Keywords: Adipocyte Progenitor Cells Perivascular Adipose Tissue Magnetic Activated Cell Sorting Adipogenesis Adipose Tissue Isolation Stromal Vascular Fraction Collagenase Digestion Cell Filtration Erythrocyte Lysis
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter