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The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart
 

The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart

Article DOI: 10.3791/55849-v • 07:59 min • June 20th, 2017
June 20th, 2017 •
*1,2, *1,2, 2, 3, 3, 1, 2
1Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 2Department of Molecular Cardiology, Heinrich Heine University, 3Department of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Heinrich Heine University
* These authors contributed equally

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résumé
Automatic Translation

This protocol describes a method to collect cardiac interstitial fluid from the isolated, perfused rat heart. To physically separate interstitial transudate from coronary venous effluent perfusate, the Langendorff perfused heart is inverted, and the transudate (interstitial fluid) formed on the cardiac surface is collected using a soft latex cap.

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Keywords: Inverted Heart Model Interstitial Transudate Collection Isolated Rat Heart Paracrine Factors Interstitial Fluid Space Cardiac Cells Inflammation Regeneration Infarcted Myocardium Latex Heart Cover Mold Liquid Latex Silicon Tubing Cardiac Transudate Ventricular Balloon Pressure Transducer Langendorff Perfusion System
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