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Immunology and Infection

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JoVE Journal Immunology and Infection
Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4
 

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

Article DOI: 10.3791/56185-v • 09:29 min • August 21st, 2017
August 21st, 2017 •
*1,2, *1, 1,2, 3, 3, 1, 4, 1,2
1Department of Neurology, University of California, 2Program in Immunology, University of California, 3Department of Neurology and Neurological Sciences, Stanford University, 4Department of Pathology, Stanford University
* These authors contributed equally

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résumé
Automatic Translation

Here, we present a protocol to induce paralysis and opticospinal inflammation by transfer of aquaporin-4 (AQP4)-specific T cells from AQP4-/- mice into WT mice. In addition, we demonstrate how to use serial optical coherence tomography to monitor visual system dysfunction.

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Keywords: Neuromyelitis Optica Aquaporin-4 T Cells Adoptive Transfer Central Nervous System Autoimmune Disease Optical Coherence Tomography Retinal Injury Complete Freund's Adjuvant Emulsion Subcutaneous Injection
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