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Miniaturized Sample Preparation for Transmission Electron Microscopy
 

Miniaturized Sample Preparation for Transmission Electron Microscopy

Article DOI: 10.3791/57310-v • 09:04 min • July 27th, 2018
July 27th, 2018 •
*1,2, *1, *1,2, 1, 1, 1, 1, 1, 3, 1, 1
1Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, 2Swiss Nanoscience Institute, University of Basel, 3BioEM lab, Biozentrum, University of Basel
* These authors contributed equally

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résumé
Automatic Translation

An instrument and methods for the preparation of nanoliter-sized sample volumes for transmission electron microscopy is presented. No paper-blotting steps are required, thus avoiding the detrimental consequences this can have for proteins, significantly reducing sample loss and enabling the analysis of single cell lysate for visual proteomics.

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Keywords: Miniaturized Sample Preparation Transmission Electron Microscopy Single Cell Analysis Visual Proteomics Protein-related Disease Cryo-EM Sample Preparation Ethane Cup Cryogen Liquid Nitrogen Ethane Gas EM Grid Microcapillary System Liquid
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