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In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy
 

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

Article DOI: 10.3791/62494-v 08:15 min September 8th, 2021
September 8th, 2021

Chapitres

résumé

Here, we present a protocol to analyze ultrastructure of the megakaryocytes in situ using transmission electron microscopy (TEM). Murine bone marrows are collected, fixed, embedded in epoxy resin and cut in ultrathin sections. After contrast staining, the bone marrow is observed under a TEM microscope at 120 kV.

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Keywords: Megakaryocytes Bone Marrow Transmission Electron Microscopy In Situ Exploration Murine Maturation Stages Ultrastructure Fixation Embedding Resin Embedding Dehydration Staining
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