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Bioengineering

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JoVE Journal Bioengineering
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
 

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Article DOI: 10.3791/57551-v • 11:56 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 3, 3, 2, 1
1Department of Biocatalysis, Institute of Chemistry, Technische Universität Berlin, 2Department of Bioenergetics, Institute of Chemistry, Technische Universität Berlin, 3Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Department of Molecular Genetics, University of Groningen
* These authors contributed equally

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résumé
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The protocol presents the Escherichia coli-based selective pressure incorporation of non-canonical amino acids (ncAAs) into the lactococcal antimicrobial peptide nisin. Its properties can be changed during recombinant expression via substitution with desired ncAAs in defined growth media. Resulting changes in bioactivity are mapped by growth inhibition assays and fluorescence microscopy.

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Antimicrobial Peptides Selective Pressure Non-canonical Amino Acids Ribosomally Synthesized Peptides Denatured Peptides Antimicrobial Activity Agar Diffusion Assays Mode Of Action Resistance Development Variety Of Peptides MRSA Pathogens Antimicrobial Agents Genetic Level Changes Modified Peptides LB Medium Antibiotics Glucose Erlenmeyer Flask Bacterial Cells
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