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Immunology and Infection

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JoVE Journal Immunology and Infection
Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice
 

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Article DOI: 10.3791/58110-v • 10:01 min • October 17th, 2018
October 17th, 2018 •
*1,2, *2, *3, 1,2, 4, 2, 5, 6, 3, 1,2,4,6, 1,2
1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 2NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 3State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, 4Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, 5Laboratory Animal Center, Chinese Center for Disease Control and Prevention, 6CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
* These authors contributed equally

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résumé
Automatic Translation

A protocol to evaluate antigen-specific T-cell responses in the immunoprivileged organs of the Ifnar1-/- murine model for the Zika virus (ZIKV) infection is described. This method is pivotal for investigating the cellular mechanisms of the protection and immunopathogenesis of ZIKV vaccines and is also valuable for their efficacy evaluation.

Tags

Zika Virus T-cell Responses Immunoprivileged Organs Ifnar1-/- Mice Tetramer-based Detection Antigen-specific T Cells Immunopathogenesis Guillain-Barre Syndrome Testes Injury Vaccine Immunization Interferon Alpha/beta Receptor Knockout Mice Brain Testes Spleen Tetramer Staining Approach Immunoprotection Immunopathogenic Cells Retro-orbital Inoculation Focus-forming Units PBS Weight Monitoring Clinical Signs Surgical Foam Skin Incision Abdominal Muscles Extraction
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