Isolation of CD4⁺ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Here, a protocol for the isolation and characterization of CD4⁺ T-cell subsets from human peripheral blood is described. Purified CD4⁺ T-cells are analyzed by flow cytometry to determine proportions of T-follicular helper cell subsets.