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JoVE Journal Immunology and Infection
Produksjon av Pseudotyped partikler for å studere svært patogene Coronaviruses i en Biosafety nivå 2
 
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Produksjon av Pseudotyped partikler for å studere svært patogene Coronaviruses i en Biosafety nivå 2

Article DOI: 10.3791/59010-v • 08:40 min • March 1st, 2019
March 1st, 2019 •
1,2, 3, 3, 4, 3,5, 3, 1
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 5Horae Gene Therapy Center, University of Massachusetts Medical School

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Her presenterer vi en protokoll for å generere pseudotyped partikler i BSL-2 omgivelser omfatter spike protein svært patogene virus Midtøsten åndedretts syndrom og alvorlig akutt åndedretts syndrom coronaviruses. Disse pseudotyped partikler inneholder et luciferase reporter gen slik at kvantifisering av viruset inn i vert målcellene.

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Kreftforskning problemet 145 Pseudotyped partikler pseudovirion coronavirus CoV spike protein alvorlig akutt åndedretts syndrom coronavirus SARS-CoV Midtøsten åndedretts syndrome coronavirus MERS-CoV murine leukemivirus MLV
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