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Developmental Biology

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JoVE Journal Developmental Biology
Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
 

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Article DOI: 10.3791/59313-v • 11:47 min • April 3rd, 2019
April 3rd, 2019 •
1,2, 3,4, 3,5,6, 7, *1,4,7, *8
1Griffith Institute for Drug Discovery, Griffith University, 2Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science, University of Sydney, 4Bosch Institute, University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit, St. Vincent's Centre for Applied Medical Research, 6School of Medical Sciences, The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science, Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health, University of Melbourne
* These authors contributed equally

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résumé
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Providing single-cell sensitivity, real-time flow cytometry is uniquely suited to quantify multimodal receptor functions of live cultures. Using adult neural progenitor cells, the P2X7 receptor function was assessed via calcium influx detected by calcium indicator dye, transmembrane pore formation by ethidium bromide uptake, and phagocytosis using fluorescent latex beads.

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Keywords: Real-time Live-cell Flow Cytometry Calcium Influx Pore Formation Phagocytosis P2X7 Receptors Neural Progenitor Cells Subventricular Zone Hippocampus Neurospheres Cell Culture Calcium Indicator Dye Calcium-free Sodium Medium Potassium Medium
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