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Developmental Biology

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JoVE Journal Developmental Biology
CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors
 

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

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résumé
Automatic Translation

Here, we present a Cas9-based exon23 deletion protocol to restore dystrophin expression in iPSC from Dmdmdx mouse-derived skin fibroblasts and directly differentiate iPSCs into myogenic progenitor cells (MPC) using the Tet-on MyoD activation system.

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CRISPR/Cas9 Technology Restoring Dystrophin Expression IPSC-derived Muscle Progenitors Duchenne Muscular Dystrophy Gene Editing Stem Cells Regeneration Autologous Therapy Genetic Correction Congenital Diseases Cell Reprogramming Gene Editing Technique
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