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Biochemistry

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JoVE Journal Biochemistry
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
 

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Article DOI: 10.3791/59842-v • 07:12 min • April 24th, 2020
April 24th, 2020 •
1, 1, 2, 2, 2, 1
1Advanced Clinical Biosystems Institute, Smidt Heart institute, Cedars Sinai Medical Center, 2Beckman Coulter Life Sciences

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Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.

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Plasma Sample Preparation Mass Spectrometry Automated Workstation Protein Enzymatic Digestion Biomarker Discovery Validation Clinical Application Accuracy Reliability Throughput Intra-assay CV Inter-assay CV High Throughput Sample Preparation Disease Proteomes Tissue Biofluids Automated Proteomic Sample Preparation Workflow High Content Proteomic Analysis Dr. Qin Fu Director Of High Throughput Center At Cedar Sinai Mr. Casey Johnson Research Associate Pooled Healthy Human Plasma Liquid Handler Device Software Workstation Syringes Autosampler Plate
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