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JoVE Journal Biochemistry
Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot
 

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

Article DOI: 10.3791/63092-v • 10:12 min • October 28th, 2021
October 28th, 2021 •
1,3, 1,3, 1,3, 1,3, 1,2,3
1Department of Medicine-Cardiology, University of Colorado Anschutz Medical Campus, 2Department of Biochemistry & Molecular Genetics, University of Colorado Anschutz Medical Campus, 3Consortium for Fibrosis Research & Translation, School of Medicine, University of Colorado Anschutz Medical Campus

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Detailed protocol and three Python scripts are provided for operating an open-source robotic liquid handling system to perform semi-automated protein sample preparation for mass spectrometry experiments, covering detergent removal, protein digestion, and peptide desalting steps.

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Shotgun Proteomics Sample Processing Automated Open-source Lab Robot Protocol Protein Biochemistry Mass Spectrometry Experiments Higher Throughput Lower Variability Proteomic Studies Low-cost Liquid Handling System Python Scripts Milton Amaya Master's Student NoSP3 Digestion.py Script Opentrons App Labware Pipettes Ammonium Bicarbonate Solution Protein Sample Two-milliliter Protein Low-bind Tubes Aluminum Block Temperature Module DTT Solution
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