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JoVE Journal Cancer Research
In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
 

In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

Article DOI: 10.3791/59849-v • 11:07 min • August 14th, 2019
August 14th, 2019 •
1, 1, 1
1Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland

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The aim of this method is to generate an in vivo model of tumor angiogenesis by xenografting mammalian tumor cells into a zebrafish embryo that has fluorescently-labelled blood vessels. By imaging the xenograft and associated vessels, a quantitative measurement of the angiogenic response can be obtained.

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Keywords: In Vivo Imaging Tumor Xenografts Zebrafish Angiogenesis Vascularization Quantitation B16-F1 Cells Microinjection Transgenic Embryos ECM Yolk Sac
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