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Immunology and Infection

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JoVE Journal Immunology and Infection
Konstruksjon av mutanter i Serotype 1 Streptococcus pneumoniae stamme 519/43
 
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Konstruksjon av mutanter i Serotype 1 Streptococcus pneumoniae stamme 519/43

Article DOI: 10.3791/61594-v • 06:06 min • September 11th, 2020
September 11th, 2020 •
1, 2, 3, 2, 1
1Faculty of Infections and Tropical Diseases, Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Division of Medicine, University College London, 3Division of Infection and Immunity, University College London UCL

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Her beskriver vi en S. pneumoniae serotype 1-stamme 519/43 som kan genmodifiseres ved å bruke sin evne til naturlig å erverve DNA og et selvmordsplasmid. Som prinsippbevis ble det laget en isogen mutant i pneumolysin (ply)-genet.

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Immunologi og infeksjon utgave 163 Serotype 1 S. pneumoniae Mutagenese naturlig kompetanse Meningittbelte
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