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Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy
 

Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy

Article DOI: 10.3791/61857-v • 09:52 min • September 21st, 2021
September 21st, 2021 •
1, 1, 1, , 1
1Oregon Hearing Research Center, Department of Otolaryngology/Head & Neck Surgery, Oregon Health & Science University

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résumé
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An open vessel-window approach using fluorescent tracers provides sufficient resolution for cochlear blood flow (CoBF) measurement. The method facilitates the study of structural and functional changes in CoBF in mouse under normal and pathological conditions.

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Keywords: Cochlear Blood Flow Meniere's Disease Hearing Loss Fluorescence Microscopy Transgenic Mouse Model Cardiac Puncture Blood Cell Labeling Surgical Procedure Anesthesia Vital Signs Monitoring
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