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Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
 

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells

Article DOI: 10.3791/61957-v • 10:00 min • December 11th, 2020
December 11th, 2020 •
1,2, 1,2, 1,2, 1, 3, 1,2
1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Max Delbrück Center for Molecular Medicine

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We present a protocol for the development and use ofan oxidative stress-model by treating retinal pigment epithelial cells with H2O2, analyzing cell morphology, viability, density, glutathione, and UCP-2 level. It is a useful model to investigate the antioxidant effect of proteins secreted by transposon-transfected cells to treat neuroretinal degeneration.

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Keywords: Oxidative Stress Hydrogen Peroxide Sleeping Beauty Transposon Human Retinal Pigment Epithelial Cells Age-related Macular Degeneration Neurodegenerative Diseases ARPE-19 Cell Line PEDF GM-CSF Nickel NTA Protein Purification BCA Protein Assay Conditioned Medium Hydrogen Peroxide Treatment
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