Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / A Flow Cytometry-Based Cell Surface Protein…
JoVE Journal
Cancer Research

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Cancer Research
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
 

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Article DOI: 10.3791/64304-v • 10:46 min • September 13th, 2022
September 13th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1School of Medicine, Deakin University, 2Institute for Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University

Chapitres

résumé
Automatic Translation

A necessary step in anticancer aptamer development is to test its binding to the target. We demonstrate a flow cytometric-based assay to study this binding, emphasizing the importance of including a negative control aptamer and cancer cells that are positive or negative for that particular protein. 

Tags

Keywords: Flow Cytometry Cell Surface Protein Aptamer Anticancer Selectivity Specificity Immunophenotyping Multi-parametric Cell Culture Trypsinization Cell Counting Cell Stabilization Blocking Buffer Aptamer Folding
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter