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JoVE Journal Cancer Research
Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells
 

Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells

Article DOI: 10.3791/56176-v • 10:28 min • January 10th, 2018
January 10th, 2018 •
1, 1, 2, 1,3, 1
1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine

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An efficient screening protocol is presented for the identification of small molecules that promote astroglial differentiation in glioblastoma stem cells (GSCs). The assay is based on a stem cell differentiation reporter whereby the expression of the enhanced GFP (eGFP) is driven by the human GFAP promoter.

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Flow Cytometry Drug Screening Small Molecules Cellular Differentiation Glioblastoma Stem Cells Astroglial Differentiation Signaling Pathways Cancer Stem Cells Glioma Stem Cells High Throughput Approach Lentivirus Particles Bloodborne Pathogens
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