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Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
 

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Article DOI: 10.3791/64945-v • 09:23 min • January 5th, 2024
January 5th, 2024 •
1, 2, 2, 2, 1, 2, 1
1Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary and Ecological Sciences (IVES), University of Liverpool, 2School of Science, Engineering, and Environment, University of Salford

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This protocol enables the impact of prophages on their hosts to be revealed. Bacterial cultures are synchronized using conditions that best support the lysogenic state, limiting spontaneous induction. RT-qPCR unequivocally distinguishes prophage-restricted genes and those uncoupled from phage control from those that are expressed during the lytic replication cycle.

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Keywords: Temperate Bacteriophages Prophages Transcriptomic Approach Bacterial Hosts Lysogen Lytic Cycle Gene Expression Bacterial Pathogens Therapeutic Targets RNA Integrity Indicator Host Lysogens Infective Phage Particles Rifampicin Resistant Top Agar Layer
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