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Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
 

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

Article DOI: 10.3791/1924-v • 08:10 min • July 19th, 2010
July 19th, 2010 •
1, 1,2
1Department of Ophthalmology, University of Pittsburgh, 2Department of Microbiology and Molecular Genetics, University of Pittsburgh

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We demonstrate a protocol to generate chimeric zebrafish embryos for live imaging cellular behavior during embryogenesis.

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Transplantation GFP-expressing Blastomeres Live Imaging Retinal Development Brain Development Chimeric Zebrafish Embryos Cell-cell Interactions Genetic Background Embryonic Transparency Morphogenesis Tissues And Organs Cellular Level Transplantation Needles GFP-negative Hosts Confocal Microscopy Embryonic Development GFP Labeling
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