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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
 

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

Article DOI: 10.3791/52465-v • 08:52 min • February 17th, 2015
February 17th, 2015 •
1, 1, 1
1Reference and Translation Center for Cardiac Stem Cell Therapy, University of Rostock

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This protocol describes how to produce functional sinus nodal tissue from murine pluripotent stem cells (PSC). T-Box3 (TBX3) overexpression plus cardiac Myosin-heavy-chain (Myh6) promoter antibiotic selection leads to highly pure pacemaker cell aggregates. These “Induced-sinoatrial-bodies” (“iSABs”) contain over 80% pacemaker cells, show highly increased beating rates and are able to pace myocardium ex vivo.

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Murine Cardiac Pacemaker Cell Aggregates ES-cell Programming Myh6-promoter-selection Sick Sinus Syndrome Artificial Pacemakers Biological Pacemakers PSCs Embryoid Bodies (EBs) Forward Programming Sinus Node Inducer TBX3 Antibiotic Selection Induced-sinoatrial-bodies (iSABs) Physiologically Functional Pacemaker Cells Nodal Cells Murine Myocardium Ex Vivo In Vitro Drug Testing Heart Tissue Engineering
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