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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
 

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Article DOI: 10.3791/54774-v • 11:57 min • December 1st, 2016
December 1st, 2016 •
1, 2,3
1Department of Biology, South University of Science and Technology of China, Shenzhen, 2Mechanobiology Institute, Singapore, 3Department of Biomedical Engineering, National University of Singapore

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We present a protocol for the application of interferometric PhotoActivated Localization Microscopy (iPALM), a 3-dimensional single-molecule localization super resolution microscopy method, to the imaging of the actin cytoskeleton in adherent mammalian cells. This approach allows light-based visualization of nanoscale structural features that would otherwise remain unresolved by conventional diffraction-limited optical microscopy.

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Three-dimensional Super Resolution Microscopy F-actin Filaments Interferometric PhotoActivated Localization Microscopy (iPALM) Actin Cytoskeleton Ultrastructural Level Nanometer Spatial Resolution Fluorescence Probes Labeling Specificity Single Molecule Localization Cover Glasses Six Well Plate Imaging Sample Buffer Well Lens Paper Rinsing Step Imaging Buffer Fast Curing Epoxy
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