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Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing
 

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

Article DOI: 10.3791/56799-v • 08:34 min • January 2nd, 2018
January 2nd, 2018 •
*1, *1, 2, 1
1Laboratorio de Oncología Molecular y TGF-β, IMIB-Arrixaca, 2Departamento de Enfermería, Facultad Enfermería, Universidad de Murcia
* These authors contributed equally

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This manuscript describes how incision-like lesions made on cultured epithelial cell monolayers conveniently model wound healing in vitro, allowing for imaging by confocal or laser scanning microscopy, and which can provide high-quality quantitative and qualitative data for studying both cell behavior and the mechanisms involved in migration.

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Microscopy Assessment Epithelial Cell Migration In Vitro Wound Healing Artificial Wounding Procedures Cell Migration Quantitative Qualitative Specific Treatments Wound Healing Molecular Markers Clinical Wounds Drug Testing Wound Closure Consistent Reproducibility Scratch Assay Culture Plate Epithelial Cells Complete Medium Serum-free Medium Sterile Microscopy
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