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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
 

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Article DOI: 10.3791/60297-v • 08:34 min • January 8th, 2020
January 8th, 2020 •
1, 1, 1,2
1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics, Joan C. Edwards School of Medicine at Marshall University

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Here, we describe a simple and reproducible protocol of mouse model of infection to evaluate the attenuation of the genetically modified strains of Pseudomonas aeruginosa in comparison to the United States Food and Drug Administration (FDA)-approved Escherichia coli for commercial applications.

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Gene Deletion Mutants Pseudomonas Aeruginosa Virulence Attenuation Mouse Model Of Infection Genome Mutations Reproducibility Mouse Infection Model Crossover Recombinant Animal Work Pathogens Clone Validation Visual Demonstration Pseudomonas Isolation Broth PIB
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