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Bioengineering

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JoVE Journal Bioengineering
Encapsulation rapide du cytosquelette reconstitué à l’intérieur de vésicules unilamellaires géantes
 
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Encapsulation rapide du cytosquelette reconstitué à l’intérieur de vésicules unilamellaires géantes

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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Cet article présente une méthode simple pour la production rapide de vésicules unilamellaires géantes avec des protéines cytosquelettiques encapsulées. La méthode s’avère utile pour la reconstitution ascendante des structures cytosquelettiques dans le confinement et les interactions cytosquelette-membrane.

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Bioingénierie Numéro 177 Transfert d’émulsion cDICE GUV reconstitution ascendante actine fascine
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