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Biochemistry

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JoVE Journal Biochemistry
Fremme af højopløsningsbilleddannelse af virussamlinger i væske og is
 
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Fremme af højopløsningsbilleddannelse af virussamlinger i væske og is

Article DOI: 10.3791/63856-v • 08:31 min • July 20th, 2022
July 20th, 2022 •
*1,2, *1, *1,3, 1,4, 1,4, 5, 6, 1, 7, 8, 2, 9, 1,5,6
1Department of Biomedical Engineering, Pennsylvania State University, 2Department of Materials Science and Engineering, McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 5Materials Research Institute, Pennsylvania State University, 6Huck Institutes of the Life Sciences, Pennsylvania State University, 7Applications team, Direct Electron, 8Application Scientist, Protochips, Inc., 9Department of Biology, Wake Forest University
* These authors contributed equally

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Her beskrives protokoller til fremstilling af virussamlinger, der er egnede til væske-EM- og kryo-EM-analyse på nanoskala ved hjælp af transmissionselektronmikroskopi.

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Biokemi udgave 185 transmissionselektronmikroskopi TEM væske-EM kryo-EM adeno-associeret virus SARS-CoV-2 rotavirus dobbeltlagspartikler DLP'er strukturel biologi
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