Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest.
Rozovski, U., Hazan-Halevy, I., Calin, G., Harris, D., Li, P., Liu, Z., Keating, M. J., Estrov, Z. Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome. J. Vis. Exp. (112), e53300, doi:10.3791/53300 (2016).