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Immunology and Infection

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JoVE Journal Immunology and Infection
Biolistischen Transformation einer Leuchtstoff Tagged-Gens in den Opportunistic Pilzpathogenentwicklung
 
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Biolistischen Transformation einer Leuchtstoff Tagged-Gens in den Opportunistic Pilzpathogenentwicklung

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Biolistische Transformation ein Verfahren verwendet werden, um eine stabile Integration der DNA in das Genom der opportunistisches Pathogen Cryptococcus neoformans durch homologe Rekombination zu erzeugen. Wir biolistische Transformation eines Konstrukts, das den kodierenden Gens Acetatkinase zur Fluoreszenzmarkierung mCherry in C. neoformans fusioniert hat demonstrieren.

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Molecular Biology Ausgabe 97 Gen-Unterbrechung, Biolistischen Lieferung Acetatkinase überlappende PCR Fluoreszenz-
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