Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / Optimizing the Genetic Incorporation of Chemical…
JoVE Journal
Chemistry

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Chemistry
Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
 

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Article DOI: 10.3791/57069-v • 14:02 min • April 9th, 2018
April 9th, 2018 •
*1, *1, *1, 1
1Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig
* These authors contributed equally

챕터

요약
Automatic Translation

A facile fluorescence assay is presented to evaluate the efficiency of amino-acyl-tRNA-synthetase/tRNA pairs incorporating non-canonical amino-acids (ncAAs) into proteins expressed in mammalian cells. The application of ncAAs to study G-protein coupled receptors (GPCRs) is described, including photo-crosslinking mapping of binding sites and bioorthogonal GPCR labeling on live cells.

Tags

Keywords: GPCR Photo-crosslinking Bioorthogonal Chemistry Non-canonical Amino Acids Amber Codon Suppression Fluorescence Assay Mammalian Cells Protein-protein Interactions Ligand Binding Live Cell Imaging
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter