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Bioengineering

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JoVE Journal Bioengineering
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
 

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Article DOI: 10.3791/59173-v • 09:57 min • April 1st, 2019
April 1st, 2019 •
*1,2, *1,3, 1,2, 4, 5, 3,6, 1,3
1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital
* These authors contributed equally

챕터

요약
Automatic Translation

Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.

Tags

Keywords: Tissue-engineered Tracheal Graft Mouse Model Orthotopic Tracheal Replacement Airway Regeneration Scaffold Electrospinning Polyethylene Terephthalate (PET) Polyurethane (PU) Microsurgical Techniques Anesthesia Graft Handling Suture Placement
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