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JoVE Journal Bioengineering
Aussaat und Implantation von einem biosynthetischen Tissue-Engineering trachealen Transplantat in einem Mausmodell
 
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Aussaat und Implantation von einem biosynthetischen Tissue-Engineering trachealen Transplantat in einem Mausmodell

Article DOI: 10.3791/59173-v • 09:57 min • April 1st, 2019
April 1st, 2019 •
*1,2, *1,3, 1,2, 4, 5, 3,6, 1,3
1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital
* These authors contributed equally

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요약
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Transplantat Stenose stellt eine kritische Hindernis Gewebezüchtungen Atemwege Ersatz. Um zellulären Mechanismen, die Stenose zu untersuchen, verwenden wir ein Mausmodell entwickelt trachealen Gewebeersatz mit kernigen Knochenmark mononukleären Zellen (BM-MNC). Hier zeigen wir unsere Protokoll, einschließlich Gerüst Herstellung, BM-MNC Isolierung Transplantat Aussaat und Implantation.

Tags

Biotechnik Ausgabe 146 Elektrospinnen synthetischen Gerüst Medizin trachealen Transplantat Zelle Aussaat Biomaterialien trachealen Krankheiten
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