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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow Cytometric Analyse der Lymphozyteninfiltration im Zentralnervensystem während der experimentellen Autoimmunenzephalomyelitis
 
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Flow Cytometric Analyse der Lymphozyteninfiltration im Zentralnervensystem während der experimentellen Autoimmunenzephalomyelitis

Article DOI: 10.3791/61050-v • 09:01 min • November 17th, 2020
November 17th, 2020 •
1,2, 1, 3, 1, 3
1Translational Medicine Research Center, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology, Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine

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Dieses Manuskript stellt ein Protokoll zur Induzieren von aktiver experimenteller Autoimmunenzephalomyelitis (EAE) bei Mäusen dar. Eine Methode zur Isolierung und Charakterisierung der infiltrierten Lymphozyten im Zentralnervensystem (ZNS) wird ebenfalls vorgestellt, um zu zeigen, wie Lymphozyten an der Entwicklung einer CNS-Autoimmunerkrankung beteiligt sind.

Tags

Immunologie und Infektion Ausgabe 165 Multiple Sklerose MS experimentelle Autoimmunenzephalomyelitis EAE Autoimmunerkrankung Zentralnervensystem ZNS T-Lymphozyten Durchflusszytometrie
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