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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
 
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In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

Article DOI: 10.3791/61068-v • 09:19 min • May 24th, 2020
May 24th, 2020 •
1, 2,3, 4, 1, 3, 1, 1,5,6
1Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group, University of Twente, 3Applied Stem Cell Technologies Group, University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, 5Institute of Physiology, Charité-Universitätsmedizin, 6German Heart Center

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We presenteren een in vitro vasculaire ziekte model om hele bloed interacties met patiënt-afgeleide endotheel te onderzoeken. Dit systeem maakt het mogelijk om tromboogene eigenschappen van primaire endotheelcellen onder verschillende omstandigheden te bestuderen. De methode is vooral geschikt om in situ tromboïgeniteit en antistollingstherapie te evalueren tijdens verschillende fasen van stolling.

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Immunologie en infectie probleem 159 trombose chronische trombo-embolische pulmonale hypertensie endotheelcellen bloedplaatjes fibrine stolling bloed
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