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Cancer Research

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Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
 

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Article DOI: 10.3791/61470-v • 09:08 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1
1Department of Dermatology, University of Arkansas for Medical Sciences

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요약
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We present a simple protocol for the isolation of peripheral blood mononuclear cells from whole blood obtained from patients diagnosed with Sézary Syndrome, followed by selection of CD4+ T cells, their stimulation with phorbol12-myristate13-acetate and A23187 ionophore, and preparation of RNA for transcriptomic profiling.

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Isolating Peripheral Blood Mononuclear Cells CD4+ T Cells S& 233;zary Syndrome Patients Transcriptomic Profiling Lymphocytes Molecular Defect Immunologic Defect Viable Cells Disease Abnormalities Purification Of RNA DNA Protein Characterize Molecular Defects Pathways Abnormalities Pathogenesis Isolation Of Peripheral Blood Fractionated Study Immune Abnormalities Alanna Medical Student Research Fellow Laboratory Blood Sample Anticoagulant Transfer Blood Separation Tubes Subject Number Dilute Samples HBSS Density Medium Layers Centrifuge
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