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JoVE Journal Neuroscience
Sezieren der zellautonomen Funktion des fragilen X-mentalen Retardierungsproteins in einem auditorischen Schaltkreis durch In-Ovo-Elektroporation
 
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Sezieren der zellautonomen Funktion des fragilen X-mentalen Retardierungsproteins in einem auditorischen Schaltkreis durch In-Ovo-Elektroporation

Article DOI: 10.3791/64187-v • 11:10 min • July 6th, 2022
July 6th, 2022 •
*1, *2, 3, 1
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, 2Department of Clinical Pathology, First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University
* These authors contributed equally

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Mit Hilfe der In-Ovo-Elektroporation entwickelten wir eine Methode, um das auditorische Innenohr und den Cochlea-Kern in Hühnerembryonen selektiv zu transfizieren, um einen zellgruppenspezifischen Knockdown des fragilen X-Mentalretardationsproteins während diskreter Perioden des Schaltkreisaufbaus zu erreichen.

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Neurowissenschaften Ausgabe 185 fragiles X-Syndrom sensorischer Schaltkreis Innenohr Cochlea-Kern Synapsenbildung Gehirnentwicklung Endbulb von Held Hühnerembryo
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