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Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
 

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

Article DOI: 10.3791/2513-v • 10:48 min • February 21st, 2011
February 21st, 2011 •
1,2, 1, 1
1Department of Medicine, Pulmonary and Critical Care Medicine, Brigham and Women’s Hospital, Harvard Medical School, 2Cellular and Molecular Pathology, School of Medicine, University of Pittsburgh

Capítulos

Resumo
Automatic Translation

Pulmonary epithelial cells can be isolated from the respiratory tract of mice and cultured at air-liquid interface as a model of differentiated respiratory epithelium. A protocol is described for isolating, culturing and exposing these cells to mainstream cigarette smoke, in order to study molecular responses to this environmental toxin.

Tags

Mouse Respiratory Epithelial Cells Isolation Experimental Cigarette Smoke Air-liquid Interface (ALI) Differentiated Respiratory Epithelium Mainstream Cigarette Smoke (CS) Exposure Airway Epithelial Cells Trachea Fully Differentiated Epithelial Cells Calibrated Mainstream CS Delivery ALI Culture System Respiratory Epithelia Physiological Setting Molecular Responses Lung Cellular Responses Environmental Air Pollution Human Health Chronic Obstructive Pulmonary Disease (COPD) Tobacco-related Diseases
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