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Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
 

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

Article DOI: 10.3791/2797-v • 13:54 min • October 10th, 2011
October 10th, 2011 •
1, 1, 1
1Department of Genetics, University of Georgia

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This whole mount in situ hybridization protocol discusses critical steps that ensure reproducible high quality results for gene expression studies in E8.5-E11.5 day old mouse embryos.

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Whole Mount In Situ Hybridization Gene Expression Patterns Embryos Probe Labeling Quality Control DIG-labeled RNA Probes In Vitro Transcription PCR Sensitivity Endogenous MRNA Fixation Storage Proteinase K Digestion Hybridization Conditions Post-hybridization Washes RNase Treatment Non-specific Probe Hybridization AP-conjugated Antibody
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