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Double Whole Mount in situ Hybridization of Early Chick Embryos
 

Double Whole Mount in situ Hybridization of Early Chick Embryos

Article DOI: 10.3791/904-v • 15:42 min • October 27th, 2008
October 27th, 2008 •
1, 2
1Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology - Texas A&M Health Science Center, 2Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)

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Resumo
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This video demonstrates 2-color whole mount in situ hybridization, a method by which the spatial and temporal expression pattern of 2 different genes can be visualized in young chick embryos. This method was originally introduced by David Wilkinson, Domingos Henrique, Phil Ingham and David Ish -Horowicz.

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Double Whole Mount In Situ Hybridization Early Chick Embryos Embryonic Development Gene Expression Brain Development Neural Tube Formation Somite Formation Heart Primordia Formation 2-color Whole Mount In Situ Hybridization Embryo Dissection Paraformaldehyde Fixation Prehybridization Probe Hybridization DIG Probe FITC Probe Overnight Hybridization DIG Coupled Antibody FITC Coupled Antibody Color Reaction For DIG Substrate Color Reaction With FITC Photograph And Sectioning Troubleshooting Guide
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