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A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
 

A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana

Article DOI: 10.3791/50732-v • 08:52 min • November 9th, 2013
November 9th, 2013 •
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1Département de Botanique et de Biologie Végétale, Université de Genève

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We present the procedure to assemble a seed coat bedding assay (SCBA) from Arabidopsis thaliana seeds. The SCBA was shown to be a powerful tool to explore genetically and in vitro how the endosperm controls seed germination in dormant seeds and in response to light cues. The SCBA is in principle applicable under any situation where the endosperm is suspected to influence embryonic growth.

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Seed Coat Bedding Assay Genetically Explore In Vitro Endosperm Seed Germination Arabidopsis Thaliana Single Cell Layer Mature Embryo Prevent Germination Dormant Seeds Far Red Light Pulse Molecular Genetic Mechanisms Germination Repressive Activity Seed Coat Bedding Assay (SCBA) Dissection Procedure Seed Coats And Embryos Monitoring Growth Of Embryos Underlying Layer Of Seed Coats Reconstitute Germination Repressive Activities Seed Dormancy FR-dependent Control Of Seed Germination Combinatorial Use Genetically Modified Seed Coat And Embryonic Materials
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