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Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
 

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Article DOI: 10.3791/2327-v • 09:23 min • January 28th, 2011
January 28th, 2011 •
1, 1, 1, 1, 1, 1
1Department of Biology, Saint Louis University

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Imprinting is a phenomenon in plant and mammal reproduction. DNA methylation plays an important role in mechanisms of imprinting. Isolating endosperm and determining methylation status of imprinted genes in Arabidopsis can be difficult. In this protocol, we describe how to isolate endosperm and determine methylation by bisulfite sequencing.

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DNA Methylation Imprinted Genes Arabidopsis Endosperm Epigenetic Mechanisms DNA Methyltransferases Loss Of DNA Methylation Imprinting Maternal And Paternal Alleles Reproduction Development Flowering Plants Double Fertilization Event MEDEA Gene FWA Gene DNA Methylation And Demethylation Imprinting Status Methylation Pattern Isolate Endosperm Genetic Cross
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