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JoVE Journal Neuroscience
A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
 

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

Article DOI: 10.3791/51219-v • 09:37 min • June 15th, 2014
June 15th, 2014 •
1, 1, 1
1Regenerative Medicine Institute, Cedars-Sinai Medical Center

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Resumo
Automatic Translation

This protocol describes a novel mechanical chopping method that allows the expansion of spherical neural stem and progenitor cell aggregates without dissociation to a single cell suspension.  Maintaining cell/cell contact allows rapid and stable growth for over 40 passages.

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Keywords: CGMP Expansion Method Neural Stem Cells Neural Progenitor Cells Pluripotent Stem Cells Fetal Brain Tissue Neurospheres Cell Culture Mechanical Passaging Automated Chopping Suspension Culture Cell Yield Scale-up Clinical-grade Cell Production
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