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Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions
 

Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

Article DOI: 10.3791/52158-v • 10:48 min • November 26th, 2014
November 26th, 2014 •
1, 1, 1,2
1Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 2Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles (UCLA)

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We describe a protocol for deriving lentiviral-based reprogrammed and characterized factor-free human induced pluripotent stem cells and conversion into putative clinical-grade conditions.

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Transgene-free Human Induced Pluripotent Stem Cells HiPSCs Lentiviral-based Reprogramming Oncogenic Genes Therapeutic Applications Non-human Antigens Clinical-grade Gene Expression Abnormalities Quantitative Polymerase Chain Reaction (PCR) Good Manufacturing Practice (GMP) Immunogenicity Risk
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