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Immunology and Infection

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JoVE Journal Immunology and Infection
High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
 

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

Article DOI: 10.3791/52195-v • 09:22 min • November 27th, 2014
November 27th, 2014 •
1,3, 1,2
1Department of Pharmacology, University of Minnesota, 2Department of Surgery, University of Minnesota, 33M Corporate Research Laboratory

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Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.

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High Throughput Fluorometric Technique Assessment Macrophage Phagocytosis Actin Polymerization Fluorometric Analysis Cell Types Adherent Cells Non-adherent Cells Phagocytic Cell Types Macrophages Fluorescently Labeled Opsonized Particles Trypan Blue 96-well Plates Fluorescent Particles Phagocytose Varied Amounts Of Time Internalization Of Fluorescent Particles Trypan Blue Wash PBS Wash Fixation DAPI Staining Fluorometric Quantification Plate Reading Relative Fluorescence Units Phagocytic Index
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