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Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
 

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Article DOI: 10.3791/52619-v • 11:28 min • March 13th, 2015
March 13th, 2015 •
1, 1, 1
1Institute of Virology, National Reference Centre for Hepatitis C, Essen University Hospital, University of Duisburg-Essen

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The preparing and processing of dried blood spots (DBS) for their final analysis are still poorly standardized for most diagnostic applications. To overcome this shortcoming, a comprehensive step-by-step protocol is suggested and subsequently evaluated with regard to its effectiveness for detecting markers of viral infections.

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Keywords: Dried Blood Spots DBS Immunoassays Molecular Techniques Diagnostic Infectious Diseases Newborn Screening Pre-analytical Variables Sample Collection Sample Preparation Sample Drying Sample Storage Sample Transportation Sample Elution Hepatitis B Hepatitis C HIV Automated Analytical Platform
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