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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
 

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

Article DOI: 10.3791/52651-v • 09:26 min • February 20th, 2015
February 20th, 2015 •
*1, *2, 2, 1, 1, 2, 1
1Departments of Pediatrics and Pharmacology, University of Montréal, 2Department of Pulmonology, Chongqing Southwest Hospital, Third Military Medical University
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.

Tags

Lymphocytic Microparticles Proapoptotic Effect Airway Epithelial Cells Cell Membrane-derived Vesicles MPs Plasma Membrane Shedding T Lymphocyte-derived Microparticles Actinomycin D Differential Centrifugation Flow Cytometry Cell Death Detection Bronchial Epithelial Cells Respiratory Bronchial Tissue Explants Disease Models Pharmacology Testing Toxicology Testing Airway Tract Tissue
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