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Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche
 

Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

Article DOI: 10.3791/52656-v • 11:42 min • March 15th, 2015
March 15th, 2015 •
1, 1, 1, 2, 1, 1
1Department of Pediatrics, Stanford University School of Medicine, 2Department of Orthopaedic Surgery, Stanford University School of Medicine

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Resumo
Automatic Translation

Protocols are described for studying breast cancer cell migration, proliferation and colonization in a human bone tissue explant model system.

Tags

Bone Metastasis Breast Cancer Human Femur Tissue Explant In Vitro Model Microenvironment Metastatic Niche Cancer Cell Colonization Bioluminescence Imaging Fluorescence Microscopy Flow Cytometry
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